Development of a 5' fluorogenic nuclease-based real-time PCR assay for quantitative detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis.
نویسندگان
چکیده
A 5' nuclease TaqMan PCR was developed for the quantitative detection of the periodontopathic bacteria Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The relative numbers of bacteria were measured by the comparative threshold cycle method. This simplified method is a way of obtaining the relative quantities of these organisms from specimens and of monitoring the effect of therapy.
منابع مشابه
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Akihiro Yoshida et al. (4). recently published an interesting paper describing the development of a 5 fluorogenic nucleasebased real-time PCR assay for quantitative detection of the oral pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The authors provide the description of a set of primers and probe for amplification and detection of a broad range of bacteria based ...
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Akihiro Yoshida et al. (4). recently published an interesting paper describing the development of a 5 fluorogenic nucleasebased real-time PCR assay for quantitative detection of the oral pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The authors provide the description of a set of primers and probe for amplification and detection of a broad range of bacteria based ...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 41 2 شماره
صفحات -
تاریخ انتشار 2003